LOCUS       Exported                4863 bp ds-DNA    circular SYN 10-OCT-2014
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    piG0043_regulator_lasR-luxR_new3
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 4863)
  AUTHORS   Daniel Gerngross
  TITLE     piG0043
  JOURNAL   Exported 10 Oct 2014 from SnapGene 2.5.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4863
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    11..202
                     /note="ROP"
     misc_feature    304..444
                     /note="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(615..1234)
                     /direction=LEFT
                     /note="pBR322_origin"
     oriT            1254..1362
                     /note="incP origin of transfer"
     CDS             complement(1552..2412)
                     /note="AmpR"
     promoter        complement(2413..2504)
                     /gene="bla"
                     /note="AmpR promoter"
     terminator      2577..2671
                     /note="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     promoter        2715..2749
                     /note="const. promoter (J23100)"
     RBS             2758..2769
                     /note="RBS (B0034)"
     CDS             2776..3498
                     /codon_start=1
                     /note="lasR (C0179)"
                     /translation="MALVDGFLELERSSGKLEWSAILQKMASDLGFSKILFGLLPKDSQ
                     DYENAFIVGNYPAAWREHYDRAGYARVDPTVSHCTQSVLPIFWEPSIYQTRKQHEFFEE
                     ASAAGLVYGLTMPLHGARGELGALSLSVEAENRAEANRFIESVLPTLWMLKDYALQSGA
                     GLAFEHPVSKPVVLTSREKEVLQWCAIGKTSWEISVICNCSEANVNFHMGNIRRKFGVT
                     SRRVAAIMAVNLGLITL*"
     terminator      3507..3635
                     /note="directional double term (B0015)"
     promoter        3641..3675
                     /note="const. promoter (J23100)"
     RBS             3684..3695
                     /note="RBS (B0034)"
     CDS             3702..4457
                     /codon_start=1
                     /product="LuxR-Barcode"
                     /note="luxR (C0062)"
                     /translation="MKNINADDTYRIINKIKACRSNNDINQCLSDMTKMVHCEYYLLAI
                     IYPHSMVKSDISILDNYPKKWRQYYDDANLIKYDPIVDYSNSNHSPINWNIFENNAVNK
                     KSPNVIKEAKTSGLITGFSFPIHTANNGFGMLSFAHSEKDNYIDSLFLHACMNIPLIVP
                     SLVDNYRKINIANNKSNNDLTKREKECLAWACEGKSSWDISKILGCSERTVTFHLTNAQ
                     MKLNTTNRCQSISKAILTGAIDCPYFKN*"
     terminator      4466..4594
                     /note="directional double term (B0015)"
     terminator      4606..4692
                     /gene="Escherichia coli rrnB"
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gaggcatcaa gtgaccaaac aggaaaaaac cgcccttaac atggcccgct ttatcagaag
       61 ccagacatta acgcttctgg agaaactcaa cgagctggac gcggatgaac aggcagacat
      121 ctgtgaatcg ctgcacgacc acgctgatga gctttaccgc agctgcctcg cgcgtttcgg
      181 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
      241 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
      301 gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg
      361 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
      421 gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc
      481 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
      541 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
      601 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
      661 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
      721 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
      781 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
      841 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
      901 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
      961 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     1021 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     1081 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     1141 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     1201 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggccggc ctacggccag
     1261 cctcgcagag caggattccc gttgagcacc gccaggtgcg aataagggac agtgaagaag
     1321 gaacacccgc tcgcgggtgg gcctacttca cctatcctgc ccggctgacg ccgttggata
     1381 caccaaggaa agtctacacg aaccctttgg caaaatcctg tatatcgtgc gaaaaaggat
     1441 ggatataccg aaaaaatcgc tataatgacc ccgaagcagg gttatgcagc ggaaaaggac
     1501 aagggtcgtc caaaaaaaaa ggctccaaaa ggagccttta attgtatcgg tttaccaatg
     1561 tttaatcagg ctcgcgccaa tttccgcaat ctgacggtta cgttcatcca tcgtcgcctg
     1621 gctgccggtg gtataaatca ccacaatacg gctcggttta ccatccgggc ccagcgccgc
     1681 aataatgcca cggctgccac gttcgcccgc accgctttta tccgcaataa accagccagc
     1741 cggcagcgcg ctacgcagca gcggacccgc cactttatcc gcttccatcc aatcaatcag
     1801 ttgctggcgg cttgccaggg tcagcagctc acccgtcagc agtttacgca gggtggtcgc
     1861 cattgctgcc ggcatggtgg tatcacgttc atcgttcgga atcgcttcgt tcagttccgg
     1921 ttcccaacga tccagacggg tcacatgatc gcccatgtta tgcagaaacg cggtcagttc
     1981 tttcggaccg ccaatggtcg tcagcagcag gttcgccgcg gtgttatcgc tcatggtaat
     2041 cgccgcgctg cacagttcac gcacggtcat gccatcggtc agatgttttt cggtcaccgg
     2101 gctatattcc accagatcgt tctggctata atgaatacga cggcccagtt gttcctggcc
     2161 cgcatccaca cggctcagaa ccgcaccgca cagcagcact ttaaaggtgc tcatcatcgg
     2221 aaaacgttct tccggacgaa aagattccag aattttgccg ctgttcagat ccagttcaat
     2281 atagcccacg cgcgcaccca gttgatcttc cgcatctttc actttcacca gggtttccgg
     2341 atgcgcaaac accggcaggc aaaacgccgc aaaaaacgga atcagcgcca cacgaaaatg
     2401 ctgaatgctc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     2461 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg aattaaaaaa
     2521 aagcccgctc attaggcggg ctactaattt aaatcgtaat tattggggac ccctggattc
     2581 tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct
     2641 gaggtcatta ctggatctat caacaggagt ccaagactag tcgccagggt tttcccagtc
     2701 acgacgcggc cgcattgacg gctagctcag tcctaggtac agtgctagct actagagaaa
     2761 gaggagaaat actagatggc cttggttgac ggttttcttg agctggaacg ctcaagtgga
     2821 aaattggagt ggagcgccat cctccagaag atggcgagcg accttggatt ctcgaagatc
     2881 ctgttcggcc tgttgcctaa ggacagccag gactacgaga acgccttcat cgtcggcaac
     2941 tacccggccg cctggcgcga gcattacgac cgggctggct acgcgcgggt cgacccgacg
     3001 gtcagtcact gtacccagag cgtactgccg attttctggg aaccgtccat ctaccagacg
     3061 cgaaagcagc acgagttctt cgaggaagcc tcggccgccg gcctggtgta tgggctgacc
     3121 atgccgctgc atggtgctcg cggcgaactc ggcgcgctga gcctcagcgt ggaagcggaa
     3181 aaccgggccg aggccaaccg tttcatagag tcggtcctgc cgaccctgtg gatgctcaag
     3241 gactacgcac tgcaaagcgg tgccggactg gccttcgaac atccggtcag caaaccggtg
     3301 gttctgacca gccgggagaa ggaagtgttg cagtggtgcg ccatcggcaa gaccagttgg
     3361 gagatatcgg ttatctgcaa ctgctcggaa gccaatgtga acttccatat gggaaatatt
     3421 cggcggaagt tcggtgtgac ctcccgccgc gtagcggcca ttatggccgt taatttgggt
     3481 cttattactc tctaataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
     3541 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
     3601 tggctcacct tcgggtgggc ctttctgcgt ttataattaa ttgacggcta gctcagtcct
     3661 aggtacagtg ctagctacta gagaaagagg agaaatacta gatgaaaaac ataaatgccg
     3721 acgacacata cagaataatt aataaaatta aagcttgtag aagcaataat gatattaatc
     3781 aatgcttatc tgatatgact aaaatggtac attgtgaata ttatttactc gcgatcattt
     3841 atcctcattc tatggttaaa tctgatattt caatcctaga taattaccct aaaaaatgga
     3901 ggcaatatta tgatgacgct aatttaataa aatatgatcc tatagtagat tattctaact
     3961 ccaatcattc accaattaat tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc
     4021 caaatgtaat taaagaagcg aaaacatcag gtcttatcac tgggtttagt ttccctattc
     4081 atacggctaa caatggcttc ggaatgctta gttttgcaca ttcagaaaaa gacaactata
     4141 tagatagttt atttttacat gcgtgtatga acataccatt aattgttcct tctctagttg
     4201 ataattatcg aaaaataaat atagcaaata ataaatcaaa caacgattta accaaaagag
     4261 aaaaagaatg tttagcgtgg gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat
     4321 taggttgcag tgagcgtact gtcactttcc atttaaccaa tgcgcaaatg aaactcaata
     4381 caacaaaccg ctgccaaagt atttctaaag caattttaac aggagcaatt gattgcccat
     4441 actttaaaaa ttaataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa
     4501 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact
     4561 ggctcacctt cgggtgggcc tttctgcgtt tataattaaa ggcatcaaat aaaacgaaag
     4621 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
     4681 agtaggacaa atccgccgcc ctagacagct gggcgcgccc ccgccgcatc cataccgcca
     4741 gttgtttacc ctcacaacgt tccagtaacc gggcatgttc atcatcagta acccgtatcg
     4801 tgagcatcct ctctcgtttc atcggtatca ttacccccat gaacagaaat tcccccttac
     4861 acg
//
